A highly stable and selective biosensor using modified nicotinic acetylcholine receptor (nAChR)

Mete Eray; Numan S. Dogan; Steven R. Reiken; Himawan Sutisna; Bernard J. Van Wie; Alan R. Koch; David F. Moffett; Moris Silber; William C. Davis

doi:10.1016/0303-2647(94)01511-5

A highly stable and selective biosensor using modified nicotinic acetylcholine receptor (nAChR)

Mete Eray, Numan S. Dogan, Steven R. Reiken, Himawan Sutisna, Bernard J. Van Wie, Alan R. Koch, David F. Moffett, Moris Silber, William C. Davis

Research output: Contribution to journal › Article › peer-review

Abstract

Methods for developing stable, sensitive and selective bilayer lipid membrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified with bispecific antibodies (BsAbs). When two BsAbs, attached to one nAChR, encounter antigen (Ag), channels are blocked. Sensitivity to single Ag molecules would be possible by monitoring closure of individual nAChRs.

Original language	English
Pages (from-to)	183-188
Number of pages	6
Journal	BioSystems
Volume	35
Issue number	2-3
DOIs	https://doi.org/10.1016/0303-2647(94)01511-5
State	Published - 1995
Externally published	Yes

Keywords

Bilayer lipid membranes
Biosensor
Bispecific antibodies
Microfabrication
Modified nicotinic acetylcholine receptor

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10.1016/0303-2647(94)01511-5

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@article{e0628992fef94f269cf9082916bf0bc6,

title = "A highly stable and selective biosensor using modified nicotinic acetylcholine receptor (nAChR)",

abstract = "Methods for developing stable, sensitive and selective bilayer lipid membrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified with bispecific antibodies (BsAbs). When two BsAbs, attached to one nAChR, encounter antigen (Ag), channels are blocked. Sensitivity to single Ag molecules would be possible by monitoring closure of individual nAChRs.",

keywords = "Bilayer lipid membranes, Biosensor, Bispecific antibodies, Microfabrication, Modified nicotinic acetylcholine receptor",

author = "Mete Eray and Dogan, \{Numan S.\} and Reiken, \{Steven R.\} and Himawan Sutisna and \{Van Wie\}, \{Bernard J.\} and Koch, \{Alan R.\} and Moffett, \{David F.\} and Moris Silber and Davis, \{William C.\}",

year = "1995",

doi = "10.1016/0303-2647(94)01511-5",

language = "English",

volume = "35",

pages = "183--188",

journal = "BioSystems",

issn = "0303-2647",

number = "2-3",

}

TY - JOUR

T1 - A highly stable and selective biosensor using modified nicotinic acetylcholine receptor (nAChR)

AU - Eray, Mete

AU - Dogan, Numan S.

AU - Reiken, Steven R.

AU - Sutisna, Himawan

AU - Van Wie, Bernard J.

AU - Koch, Alan R.

AU - Moffett, David F.

AU - Silber, Moris

AU - Davis, William C.

PY - 1995

Y1 - 1995

N2 - Methods for developing stable, sensitive and selective bilayer lipid membrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified with bispecific antibodies (BsAbs). When two BsAbs, attached to one nAChR, encounter antigen (Ag), channels are blocked. Sensitivity to single Ag molecules would be possible by monitoring closure of individual nAChRs.

AB - Methods for developing stable, sensitive and selective bilayer lipid membrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified with bispecific antibodies (BsAbs). When two BsAbs, attached to one nAChR, encounter antigen (Ag), channels are blocked. Sensitivity to single Ag molecules would be possible by monitoring closure of individual nAChRs.

KW - Bilayer lipid membranes

KW - Biosensor

KW - Bispecific antibodies

KW - Microfabrication

KW - Modified nicotinic acetylcholine receptor

UR - https://www.scopus.com/pages/publications/0029038564

U2 - 10.1016/0303-2647(94)01511-5

DO - 10.1016/0303-2647(94)01511-5

M3 - Article

SN - 0303-2647

VL - 35

SP - 183

EP - 188

JO - BioSystems

JF - BioSystems

IS - 2-3

ER -

A highly stable and selective biosensor using modified nicotinic acetylcholine receptor (nAChR)

Abstract

Keywords

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