A method for isolation of Pasteuria penetrans endospores for bioassay and genomic studies

Jenora T. Waterman; David Mc K. Bird; Charles H. Opperman

A method for isolation of Pasteuria penetrans endospores for bioassay and genomic studies

Jenora T. Waterman
, David Mc K. Bird
, Charles H. Opperman

Research output: Contribution to journal › Article

Abstract

A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations. © The Society of Nematologists 2006.

Original language	English
Journal	Journal of Nematology
Volume	38
Issue number	Issue 1
State	Published - 2006

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title = "A method for isolation of Pasteuria penetrans endospores for bioassay and genomic studies",

abstract = "A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations. {\textcopyright} The Society of Nematologists 2006.",

author = "Waterman, \{Jenora T.\} and Bird, \{David Mc K.\} and Opperman, \{Charles H.\}",

year = "2006",

language = "English",

volume = "38",

journal = "Journal of Nematology",

number = "Issue 1",

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T1 - A method for isolation of Pasteuria penetrans endospores for bioassay and genomic studies

AU - Waterman, Jenora T.

AU - Bird, David Mc K.

AU - Opperman, Charles H.

PY - 2006

Y1 - 2006

N2 - A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations. © The Society of Nematologists 2006.

AB - A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations. © The Society of Nematologists 2006.

M3 - Article

VL - 38

JO - Journal of Nematology

JF - Journal of Nematology

IS - Issue 1

ER -

A method for isolation of Pasteuria penetrans endospores for bioassay and genomic studies

Abstract

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