Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays

F. Lou; Y. J. Cong; Salam A Ibrahim; Fazheng Ren; L. F. Li

doi:10.1080/09540100903443709

Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays

F. Lou
, Y. J. Cong
, Salam A Ibrahim
, Fazheng Ren
, L. F. Li

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We conducted enzyme-linked immunosorbent assay (ELISA) and basophilactivation assay in order to investigate the presence of allergens in selected cow milk (CM) substitutes. Our results showed the presence of residual antigenicity and allergenicity. The cross-reactivity between proteins from different animal species was observed. CM allergen-induced histamine release was dose-dependent. Therefore, differences in the relative percentage of major allergens may lead to a discrepancy between allergenic and antigenic activity. The presence of very similar epitopes could explain the cross-reactivity in mammalian milks. © 2010 Taylor & Francis.

Original language	English
Pages (from-to)	119-130
Number of pages	12
Journal	Food and Agricultural Immunology
Volume	21
Issue number	2
DOIs	https://doi.org/10.1080/09540100903443709
State	Published - Jun 1 2010

Keywords

Allergenicity
Antigenicity
Cow milk substitute

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10.1080/09540100903443709

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title = "Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays",

abstract = "We conducted enzyme-linked immunosorbent assay (ELISA) and basophilactivation assay in order to investigate the presence of allergens in selected cow milk (CM) substitutes. Our results showed the presence of residual antigenicity and allergenicity. The cross-reactivity between proteins from different animal species was observed. CM allergen-induced histamine release was dose-dependent. Therefore, differences in the relative percentage of major allergens may lead to a discrepancy between allergenic and antigenic activity. The presence of very similar epitopes could explain the cross-reactivity in mammalian milks. {\textcopyright} 2010 Taylor \& Francis.",

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T1 - Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays

AU - Lou, F.

AU - Cong, Y. J.

AU - Ibrahim, Salam A

AU - Ren, Fazheng

AU - Li, L. F.

PY - 2010/6/1

Y1 - 2010/6/1

N2 - We conducted enzyme-linked immunosorbent assay (ELISA) and basophilactivation assay in order to investigate the presence of allergens in selected cow milk (CM) substitutes. Our results showed the presence of residual antigenicity and allergenicity. The cross-reactivity between proteins from different animal species was observed. CM allergen-induced histamine release was dose-dependent. Therefore, differences in the relative percentage of major allergens may lead to a discrepancy between allergenic and antigenic activity. The presence of very similar epitopes could explain the cross-reactivity in mammalian milks. © 2010 Taylor & Francis.

AB - We conducted enzyme-linked immunosorbent assay (ELISA) and basophilactivation assay in order to investigate the presence of allergens in selected cow milk (CM) substitutes. Our results showed the presence of residual antigenicity and allergenicity. The cross-reactivity between proteins from different animal species was observed. CM allergen-induced histamine release was dose-dependent. Therefore, differences in the relative percentage of major allergens may lead to a discrepancy between allergenic and antigenic activity. The presence of very similar epitopes could explain the cross-reactivity in mammalian milks. © 2010 Taylor & Francis.

KW - Allergenicity

KW - Antigenicity

KW - Cow milk substitute

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VL - 21

SP - 119

EP - 130

JO - Food and Agricultural Immunology

JF - Food and Agricultural Immunology

IS - 2

ER -

Allergenic and antigenic activity of cow milk substitutes by ELISA and basophil-activation assays

Abstract

Keywords

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