TY - JOUR
T1 - CAGE: Chromatin Analogous Gene Expression
AU - Zadegan, Reza
AU - Hughes, William L.
PY - 2017/10/20
Y1 - 2017/10/20
N2 - Self-assembled nucleic acids perform biological, chemical, and mechanical work at the nanoscale. DNA-based molecular machines have been designed here to perform work by reacting with cancer-specific miRNA mimics and then regulating gene expression in vitro by tuning RNA polymerase activity. Because RNA production is topologically restrained, the machines demonstrate chromatin analogous gene expression (CAGE). With modular and tunable design features, CAGE has potential for molecular biology, synthetic biology, and personalized medicine applications.
AB - Self-assembled nucleic acids perform biological, chemical, and mechanical work at the nanoscale. DNA-based molecular machines have been designed here to perform work by reacting with cancer-specific miRNA mimics and then regulating gene expression in vitro by tuning RNA polymerase activity. Because RNA production is topologically restrained, the machines demonstrate chromatin analogous gene expression (CAGE). With modular and tunable design features, CAGE has potential for molecular biology, synthetic biology, and personalized medicine applications.
KW - CAGE
KW - gene expression
KW - molecular machine
KW - RNA polymerase regulation
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85026909367&origin=inward
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85026909367&origin=inward
U2 - 10.1021/acssynbio.7b00045
DO - 10.1021/acssynbio.7b00045
M3 - Article
C2 - 28657718
SN - 2161-5063
VL - 6
SP - 1800
EP - 1806
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
IS - 10
ER -
