CAGE: Chromatin Analogous Gene Expression

Reza Zadegan; William L. Hughes

doi:10.1021/acssynbio.7b00045

CAGE: Chromatin Analogous Gene Expression

Reza Zadegan
, William L. Hughes

Joint School of Nanoscience and Nanoengineering

Boise State University
Boise State University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Self-assembled nucleic acids perform biological, chemical, and mechanical work at the nanoscale. DNA-based molecular machines have been designed here to perform work by reacting with cancer-specific miRNA mimics and then regulating gene expression in vitro by tuning RNA polymerase activity. Because RNA production is topologically restrained, the machines demonstrate chromatin analogous gene expression (CAGE). With modular and tunable design features, CAGE has potential for molecular biology, synthetic biology, and personalized medicine applications.

Original language	English
Pages (from-to)	1800-1806
Number of pages	7
Journal	ACS Synthetic Biology
Volume	6
Issue number	10
DOIs	https://doi.org/10.1021/acssynbio.7b00045
State	Published - Oct 20 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

CAGE
gene expression
molecular machine
RNA polymerase regulation

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10.1021/acssynbio.7b00045

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AB - Self-assembled nucleic acids perform biological, chemical, and mechanical work at the nanoscale. DNA-based molecular machines have been designed here to perform work by reacting with cancer-specific miRNA mimics and then regulating gene expression in vitro by tuning RNA polymerase activity. Because RNA production is topologically restrained, the machines demonstrate chromatin analogous gene expression (CAGE). With modular and tunable design features, CAGE has potential for molecular biology, synthetic biology, and personalized medicine applications.

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CAGE: Chromatin Analogous Gene Expression

Abstract

UN SDGs

Keywords

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