Determination of diarylheptanoids from Alpinia officinarum (lesser galangal) by HPLC with photodiode array and electrochemical detection

Zhihua Liu; Shengmin Sang; Thomas G. Hartman; Chi Tang Ho; Robert T. Rosen

doi:10.1002/pca.827

Determination of diarylheptanoids from Alpinia officinarum (lesser galangal) by HPLC with photodiode array and electrochemical detection

Zhihua Liu, Shengmin Sang, Thomas G. Hartman, Chi Tang Ho, Robert T. Rosen

Rutgers, Stt. Univ. of New Jersey

Research output: Contribution to journal › Article › peer-review

Abstract

Normal-phase column chromatography followed by semi-preparative reversed-phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum, five diarylheptanoids identified as 5-hydroxy-7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-3-heptanone, 5-methoxy-7-(4″-hydroxy-3″- methoxyphenyl)-1-phenyl-3-heptanone, 7-(4″-hydroxyphenyl) -1-phenylhept-4-en-3-one, 7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-hept-4-en-3-one, 1,7-diphenylhept-4-en-3-one. The levels of these five diarylheptanoids in root material were determined quantitatively by HPLC with UV detection and the assay methods so developed were simple, rapid and accurate. Four of the diarylheptanoids could also be detected by HPLC with electrochemical detection (ECD) in the oxidative mode, and ECD was found to have a higher sensitivity than photodiode array detection.

Original language	English
Pages (from-to)	252-256
Number of pages	5
Journal	Phytochemical Analysis
Volume	16
Issue number	4
DOIs	https://doi.org/10.1002/pca.827
State	Published - 2005
Externally published	Yes

Keywords

Alpinia officinarum
Diarylheptanoids
HPLC-ECD
HPLC-PAD
Lesser galangal
Zingiberaceae

Access to Document

10.1002/pca.827

Cite this

@article{def9e72554364bfe8fcad3aa1d3a468e,

title = "Determination of diarylheptanoids from Alpinia officinarum (lesser galangal) by HPLC with photodiode array and electrochemical detection",

abstract = "Normal-phase column chromatography followed by semi-preparative reversed-phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum, five diarylheptanoids identified as 5-hydroxy-7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-3-heptanone, 5-methoxy-7-(4″-hydroxy-3″- methoxyphenyl)-1-phenyl-3-heptanone, 7-(4″-hydroxyphenyl) -1-phenylhept-4-en-3-one, 7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-hept-4-en-3-one, 1,7-diphenylhept-4-en-3-one. The levels of these five diarylheptanoids in root material were determined quantitatively by HPLC with UV detection and the assay methods so developed were simple, rapid and accurate. Four of the diarylheptanoids could also be detected by HPLC with electrochemical detection (ECD) in the oxidative mode, and ECD was found to have a higher sensitivity than photodiode array detection.",

keywords = "Alpinia officinarum, Diarylheptanoids, HPLC-ECD, HPLC-PAD, Lesser galangal, Zingiberaceae",

author = "Zhihua Liu and Shengmin Sang and Hartman, \{Thomas G.\} and Ho, \{Chi Tang\} and Rosen, \{Robert T.\}",

year = "2005",

doi = "10.1002/pca.827",

language = "English",

volume = "16",

pages = "252--256",

journal = "Phytochemical Analysis",

issn = "0958-0344",

number = "4",

}

TY - JOUR

T1 - Determination of diarylheptanoids from Alpinia officinarum (lesser galangal) by HPLC with photodiode array and electrochemical detection

AU - Liu, Zhihua

AU - Sang, Shengmin

AU - Hartman, Thomas G.

AU - Ho, Chi Tang

AU - Rosen, Robert T.

PY - 2005

Y1 - 2005

N2 - Normal-phase column chromatography followed by semi-preparative reversed-phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum, five diarylheptanoids identified as 5-hydroxy-7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-3-heptanone, 5-methoxy-7-(4″-hydroxy-3″- methoxyphenyl)-1-phenyl-3-heptanone, 7-(4″-hydroxyphenyl) -1-phenylhept-4-en-3-one, 7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-hept-4-en-3-one, 1,7-diphenylhept-4-en-3-one. The levels of these five diarylheptanoids in root material were determined quantitatively by HPLC with UV detection and the assay methods so developed were simple, rapid and accurate. Four of the diarylheptanoids could also be detected by HPLC with electrochemical detection (ECD) in the oxidative mode, and ECD was found to have a higher sensitivity than photodiode array detection.

AB - Normal-phase column chromatography followed by semi-preparative reversed-phase HPLC has been used to isolate, from the rhizomes of Alpinia officinarum, five diarylheptanoids identified as 5-hydroxy-7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-3-heptanone, 5-methoxy-7-(4″-hydroxy-3″- methoxyphenyl)-1-phenyl-3-heptanone, 7-(4″-hydroxyphenyl) -1-phenylhept-4-en-3-one, 7-(4″-hydroxy-3″-methoxyphenyl) -1-phenyl-hept-4-en-3-one, 1,7-diphenylhept-4-en-3-one. The levels of these five diarylheptanoids in root material were determined quantitatively by HPLC with UV detection and the assay methods so developed were simple, rapid and accurate. Four of the diarylheptanoids could also be detected by HPLC with electrochemical detection (ECD) in the oxidative mode, and ECD was found to have a higher sensitivity than photodiode array detection.

KW - Alpinia officinarum

KW - Diarylheptanoids

KW - HPLC-ECD

KW - HPLC-PAD

KW - Lesser galangal

KW - Zingiberaceae

UR - https://www.scopus.com/pages/publications/22144472258

U2 - 10.1002/pca.827

DO - 10.1002/pca.827

M3 - Article

SN - 0958-0344

VL - 16

SP - 252

EP - 256

JO - Phytochemical Analysis

JF - Phytochemical Analysis

IS - 4

ER -

Determination of diarylheptanoids from Alpinia officinarum (lesser galangal) by HPLC with photodiode array and electrochemical detection

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this