TY - JOUR
T1 - Dopamine-Functionalized Gold Nanoparticles for Colorimetric Detection of Histamine
AU - Unabia, Romnick B.
AU - Reazo, Renzo Luis D.
AU - Rivera, Rolen Brian P.
AU - Lapening, Melbagrace A.
AU - Omping, Jahor L.
AU - Lumod, Ryan M.
AU - Ruda, Archie G.
AU - Sayson, Noel Lito B.
AU - Dumancas, Gerard
AU - Malaluan, Roberto M.
AU - Lubguban, Arnold A.
AU - Petalcorin, Gaudencio C.
AU - Capangpangan, Rey Y.
AU - Latayada, Felmer S.
AU - Alguno, Arnold C.
PY - 2024/1/1
Y1 - 2024/1/1
N2 - Histamine, a primary biogenic amine (BA) generated through the decarboxylation of amino acids, concentration increases in protein-rich foods during deterioration. Thus, its detection plays a crucial role in ensuring food safety and quality. This study introduces an innovative approach involving the direct integration of dopamine onto gold nanoparticles (DCt-AuNP), aiming at rapid histamine colorimetric detection. Transmission electron microscopy revealed the aggregation of uniformly distributed spherical DCt-AuNPs with 12.02 ± 2.53 nm sizes upon the addition of histamine to DCt-AuNP solution. The Fourier-transform infrared (FTIR) spectra demonstrated the disappearance of the dicarboxy acetone peak at 1710 cm−1 along with the formation of well-defined peaks at 1585 cm−1, and 1396 cm−1 associated with the N−H bending modes and the aromatic C=C bond stretching vibration in histamine molecule, respectively, confirming the ligand exchange and interactions of histamine on the surface of DCt-AuNPs. The UV−vis spectra of the DCt-AuNP solution exhibited a red shift and a reduction in surface plasmon resonance (SPR) peak intensity at 518 nm along with the emergence of the 650 nm peak, signifying aggregation DCt-AuNPs with increasing histamine concentration. Notably, color transitions from wine-red to deep blue were observed in the DCt-AuNP solution in response to histamine, providing a reliable colorimetric signal. Dynamic Light Scattering (DLS) characterization showed a significant increase in the hydrodynamic diameter, from ∼15 to ∼1690 nm, confirming the interparticle cross-linking of DCt-AuNPs in the presence of histamine. This newly developed DCt-AuNP sensor provides colorimetric results in less than a minute that exhibits a remarkable naked-eye histamine detection threshold of 1.57 μM and a calculated detection limit of 0.426 μM, making it a promising tool for the rapid and sensitive detection of histamine.
AB - Histamine, a primary biogenic amine (BA) generated through the decarboxylation of amino acids, concentration increases in protein-rich foods during deterioration. Thus, its detection plays a crucial role in ensuring food safety and quality. This study introduces an innovative approach involving the direct integration of dopamine onto gold nanoparticles (DCt-AuNP), aiming at rapid histamine colorimetric detection. Transmission electron microscopy revealed the aggregation of uniformly distributed spherical DCt-AuNPs with 12.02 ± 2.53 nm sizes upon the addition of histamine to DCt-AuNP solution. The Fourier-transform infrared (FTIR) spectra demonstrated the disappearance of the dicarboxy acetone peak at 1710 cm−1 along with the formation of well-defined peaks at 1585 cm−1, and 1396 cm−1 associated with the N−H bending modes and the aromatic C=C bond stretching vibration in histamine molecule, respectively, confirming the ligand exchange and interactions of histamine on the surface of DCt-AuNPs. The UV−vis spectra of the DCt-AuNP solution exhibited a red shift and a reduction in surface plasmon resonance (SPR) peak intensity at 518 nm along with the emergence of the 650 nm peak, signifying aggregation DCt-AuNPs with increasing histamine concentration. Notably, color transitions from wine-red to deep blue were observed in the DCt-AuNP solution in response to histamine, providing a reliable colorimetric signal. Dynamic Light Scattering (DLS) characterization showed a significant increase in the hydrodynamic diameter, from ∼15 to ∼1690 nm, confirming the interparticle cross-linking of DCt-AuNPs in the presence of histamine. This newly developed DCt-AuNP sensor provides colorimetric results in less than a minute that exhibits a remarkable naked-eye histamine detection threshold of 1.57 μM and a calculated detection limit of 0.426 μM, making it a promising tool for the rapid and sensitive detection of histamine.
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U2 - 10.1021/acsomega.3c10123
DO - 10.1021/acsomega.3c10123
M3 - Article
SN - 2470-1343
VL - 9
SP - 17238
EP - 17246
JO - ACS Omega
JF - ACS Omega
IS - 15
ER -