Abstract
The objective of this work was to determine the effect of media composition and incubation temperatures on autoaggregation behavior of bifidobacteria. Thirty-seven different strains of bifidobacteria were screened for autoaggregation behavior during the incubation period. Results showed that these were 3 different types of autoaggregation: (1) autoaggregation sensitive (S) for strains that formed a precipitate resulting in a clear solution; (2) autoaggregation resistant (R) for strains that produced consistent turbidity, and (3) autoaggregation moderate (M) for strains that showed slight turbidity. Results on the media composition showed that TPY broth increased the autoaggregation behavior of the tested strains, whereas both Wilkins-Chalgren and Lactobacillus MRS reduced autoaggregation behavior. Calcium ions induced the autoaggregation, whereas Tween 20 and Tween 80 reduced autoaggregation behavior. Higher incubation temperature (42°C compared to 34°C) increased the ability of strains to autoaggregate. Our results indicated that media composition and incubation temperature could be considered as important factors affecting autoaggregation behavior of bifidobacteria.
| Original language | English |
|---|---|
| Pages (from-to) | 127-129 |
| Number of pages | 3 |
| Journal | Milchwissenschaft |
| Volume | 60 |
| Issue number | 2 |
| State | Published - 2005 |
| Externally published | Yes |