Evolution of nitrogen species in landfill leachates under various stabilization states

In this study, nitrogen species in landfill leachates under various stabilization states were investigated with emphasis on organic nitrogen. Ammonium nitrogen was found to be approximately 1300 mg/L in leachates from younger landfill units (less than 10 years old), and approximately 500 mg/L in leachates from older landfill units (up to 30 years old). The concentration and aerobic biodegradability of organic nitrogen decreased with landfill age. A size distribution study showed that most organic nitrogen in landfill leachates is <1 kDa. The Lowry protein concentration (mg/L-N) was analyzed and showed a strong correlation with the total organic nitrogen (TON, mg/L-N, R2 = 0.88 and 0.98 for untreated and treated samples, respectively). The slopes of the regression curves of untreated (protein = 0.45TON) and treated (protein = 0.31TON) leachates indicated that the protein is more biodegradable than the other organic nitrogen species in landfill leachates. XAD-8 resin was employed to isolate the hydrophilic fraction of leachate samples, and it was found that the hydrophilic fraction proportion in terms of organic nitrogen decreased with landfill age. Solid-state 15N nuclear magnetic resonance (NMR) was utilized to identify the nitrogen species. Proteinaceous materials were found to be readily biodegradable, while heterocyclic nitrogen species were found to be resistant to biodegradation.