TY - GEN
T1 - Growth and transcriptional profile analysis following oral probiotic supplementation in dairy cows
AU - Worku, M
AU - Adjei-Fremah, S
AU - Ekwemalor, K
AU - Asiamah, E
AU - Ismail, H
PY - 2016
Y1 - 2016
N2 - The objective of this study was to assess the impact of probiotic administration on growth and global gene expression profile in dairy cow. Use of probiotic supplements is a nonchemical approach to promote animal health. Understanding the mechanism of action of probiotics in cows may aid in sustainable dairy production. Lactating Holstein-Friesian cows (n = 10) received daily oral doses (50ml) of a commercial probiotic FASTtrak microbial pack (Conklin Company, Kansas City, MO) (containing Lactobacillus acidophilus, Saccharomyces cerevisiae, Enterococcus faecium, Aspergillus oryza, and Bacillus subtilis) over a 60-d period. Body weight was recorded weekly. Whole blood was collected at the beginning (d 0) and end of the study (d 60). Blood samples were analyzed for total and viable cell count, packed cell volume (PCV), white blood cell differential counts (WBC), and total protein concentration in plasma. Daily supplementation of probiotics had no effect on BW, PCV, and total protein concentration in plasma at the end of the study (P > 0.05). Percentage lymphocyte count increased (P < 0.05), and percentage neutrophil count (P < 0.05) decreased in probiotic-treated animals. Gene expression analysis identified 10,859 differentially expressed genes, 1168 up-regulated and 9691 down-regulated genes respectively following probiotic administration. Pathway analysis identified 87 bovine pathways impacted by probiotic treatment. These pathways included the Toll-like receptor signaling pathway, inflammation response and Wnt signaling pathways. Oral administration of probiotic to dairy cows has a systemic effect on global gene expression, including genes involved in immunity and homeostasis (Wnt). The results of this study show that the utilization of probiotics in animal agriculture impacts genes important to dairy cow health and production. Further definition of the interaction between the pathways involved may aid in the design of the most effective probiotics for optimum dairy production and health
AB - The objective of this study was to assess the impact of probiotic administration on growth and global gene expression profile in dairy cow. Use of probiotic supplements is a nonchemical approach to promote animal health. Understanding the mechanism of action of probiotics in cows may aid in sustainable dairy production. Lactating Holstein-Friesian cows (n = 10) received daily oral doses (50ml) of a commercial probiotic FASTtrak microbial pack (Conklin Company, Kansas City, MO) (containing Lactobacillus acidophilus, Saccharomyces cerevisiae, Enterococcus faecium, Aspergillus oryza, and Bacillus subtilis) over a 60-d period. Body weight was recorded weekly. Whole blood was collected at the beginning (d 0) and end of the study (d 60). Blood samples were analyzed for total and viable cell count, packed cell volume (PCV), white blood cell differential counts (WBC), and total protein concentration in plasma. Daily supplementation of probiotics had no effect on BW, PCV, and total protein concentration in plasma at the end of the study (P > 0.05). Percentage lymphocyte count increased (P < 0.05), and percentage neutrophil count (P < 0.05) decreased in probiotic-treated animals. Gene expression analysis identified 10,859 differentially expressed genes, 1168 up-regulated and 9691 down-regulated genes respectively following probiotic administration. Pathway analysis identified 87 bovine pathways impacted by probiotic treatment. These pathways included the Toll-like receptor signaling pathway, inflammation response and Wnt signaling pathways. Oral administration of probiotic to dairy cows has a systemic effect on global gene expression, including genes involved in immunity and homeostasis (Wnt). The results of this study show that the utilization of probiotics in animal agriculture impacts genes important to dairy cow health and production. Further definition of the interaction between the pathways involved may aid in the design of the most effective probiotics for optimum dairy production and health
M3 - Conference contribution
VL - 94
SP - 61
EP - 61
BT - Unknown book
ER -