Influence of brodifacoum and bromadiolone on growth of yoghurt cultures in milk

The influence of brodifacoum and bromadiolone on the growth of yoghurt culture in milk was determined. Milk samples inoculated with a commercial yoghurt culture and serially diluted rodenticides were incubated at 42 °C for 6 h. The yoghurt cultures showed detectable sensitivity (significant change (P < 0.05) in pH in the medium) to brodifacoum at 0.12 mg/mL and to bromadiolone at 0.08 mg/mL. There were significant differences (P < 0.05) in the bacterial count and α-galactosidase and β-galactosidase activities in the presence of toxins in milk. The results indicate that brodifacoum and bromadiolone could inhibit the growth of yoghurt culture in milk.