TY - JOUR
T1 - Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein
AU - Niu, Xiaoyu
AU - Xu, Jiayu
AU - Liu, Mingde
AU - Tu, Huolin
AU - Koenig, Sara N.
AU - Saif, Linda J.
AU - Jones, Daniel M.
AU - Wang, Qiuhong
PY - 2023/1/1
Y1 - 2023/1/1
N2 - We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020‐January 2021.
AB - We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020‐January 2021.
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U2 - 10.1007/s00705-022-05621-5
DO - 10.1007/s00705-022-05621-5
M3 - Article
C2 - 36539656
SN - 0304-8608
VL - 168
JO - Archives of Virology
JF - Archives of Virology
IS - 1
M1 - 5
ER -