Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

Shanta R Bhattarai; Ho-Keun Yi; Narayan Bhattarai; Pyoung Han Hwang; Hak Yong Kim

doi:10.1016/j.actbio.2005.10.007

Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

Shanta R Bhattarai
, Ho-Keun Yi
, Narayan Bhattarai
, Pyoung Han Hwang
, Hak Yong Kim

Chemical, Biological, and Bio Engineering

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co- l -lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the β-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by β-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy. © 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	207-212
Number of pages	6
Journal	Acta Biomaterialia
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.actbio.2005.10.007
State	Published - Jan 1 2006

Keywords

Biodegradable DNA
Cell proliferation
Cell transfection
Poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol)

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10.1016/j.actbio.2005.10.007

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title = "Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection",

abstract = "The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co- l -lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the β-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by β-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy. {\textcopyright} 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.",

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doi = "10.1016/j.actbio.2005.10.007",

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T1 - Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

AU - Bhattarai, Shanta R

AU - Yi, Ho-Keun

AU - Bhattarai, Narayan

AU - Hwang, Pyoung Han

AU - Kim, Hak Yong

PY - 2006/1/1

Y1 - 2006/1/1

N2 - The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co- l -lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the β-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by β-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy. © 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

AB - The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-co- l -lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the β-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by β-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy. © 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

KW - Biodegradable DNA

KW - Cell proliferation

KW - Cell transfection

KW - Poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol)

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JO - Acta Biomaterialia

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ER -

Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

Abstract

Keywords

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