Photocatalytic inactivation efficiency of anatase nano-TiO2 sol on the H9N2 avian influenza virus

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Abstract

This study was conducted to investigate efficiency of TiO 2nanomaterial as a novel environment-friendly disinfectant to control avian influenza (AI) by its photochemical sterilization ability. Anatase nano-TiO2sol, a neutral, viscous aqueous colloid of 1.6% TiO 2, was synthesized from peroxotitanic acid solution according to the Ichinose method. Transmission electron microscope images showed that the TiO2particles were spindle-shaped with an average size of 50 nm. X-ray diffraction patterns revealed that the crystal phase of TiO 2particles was anatase type with photocatalytic effect. A photocatalytic film of nano-TiO2sol was tested as a means of inactivating H9N2avian influenza virus (AIV). Inactivation capabilities were examined with 365 nm ultraviolet (UV) radiation under black light by adjusting the UV intensity, the UV irradiation time and the quantity of AIV. The titer change of AIV was determined by hemagglutination tests. Cytopathic effect of Madin Darby canine kidney (MDCK) cells was monitored by inverted fluorescence microscope. The results showed that anatase nano-TiO 2sol significantly inactivated AIV under UV irradiation of 365 nm. The inactivation of AIV viruses reached up to 100%. Therefore, anatase nano-TiO2sol is a potentially environment-friendly antivirus agent to prevent AI. © 2010 The Authors. Journal Compilation. The American Society of Photobiology.
Original languageEnglish
Pages (from-to)1135-1139
Number of pages5
JournalPhotochemistry and Photobiology
Volume86
Issue number5
DOIs
StatePublished - Sep 1 2010

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