Preparative isolation and purification of germacrone and curdione from the essential oil of the rhizomes of Curcuma wenyujin by high-speed counter-current chromatography

Jizhong Yan; Gang Chen; Shengqiang Tong; Yeping Feng; Liuqing Sheng; Jianzhong Lou

doi:10.1016/j.chroma.2005.02.064

Preparative isolation and purification of germacrone and curdione from the essential oil of the rhizomes of Curcuma wenyujin by high-speed counter-current chromatography

Jizhong Yan, Gang Chen, Shengqiang Tong, Yeping Feng, Liuqing Sheng, Jianzhong Lou

Research output: Contribution to journal › Article › peer-review

Abstract

High-speed counter-current chromatography (HSCCC) was applied to isolate and purify bioactive compounds in the essential oil of the rhizomes of Curcuma wenyujin. Two sesquiterpenes germacrone and curdione were successfully isolated and purified using two-phase solvent system composed of petroleum ether-ethanol-diethyl ether-water (5/4/0.5/1, v/v) in tail to head elution mode. 62 mg of germacrone and 93 mg of curdione were obtained from 658 mg of the essential oil each at over 95% purity. Their structures were identified by ¹H NMR and EI MS.

Original language	English
Pages (from-to)	207-210
Number of pages	4
Journal	Journal of Chromatography A
Volume	1070
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2005.02.064
State	Published - Apr 8 2005
Externally published	Yes

Keywords

Counter-current chromatography
Curcuma wenyujin
Curdione
Germacrone
Purification

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10.1016/j.chroma.2005.02.064

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title = "Preparative isolation and purification of germacrone and curdione from the essential oil of the rhizomes of Curcuma wenyujin by high-speed counter-current chromatography",

abstract = "High-speed counter-current chromatography (HSCCC) was applied to isolate and purify bioactive compounds in the essential oil of the rhizomes of Curcuma wenyujin. Two sesquiterpenes germacrone and curdione were successfully isolated and purified using two-phase solvent system composed of petroleum ether-ethanol-diethyl ether-water (5/4/0.5/1, v/v) in tail to head elution mode. 62 mg of germacrone and 93 mg of curdione were obtained from 658 mg of the essential oil each at over 95\% purity. Their structures were identified by 1H NMR and EI MS.",

keywords = "Counter-current chromatography, Curcuma wenyujin, Curdione, Germacrone, Purification",

author = "Jizhong Yan and Gang Chen and Shengqiang Tong and Yeping Feng and Liuqing Sheng and Jianzhong Lou",

year = "2005",

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doi = "10.1016/j.chroma.2005.02.064",

language = "English",

volume = "1070",

pages = "207--210",

journal = "Journal of Chromatography A",

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TY - JOUR

T1 - Preparative isolation and purification of germacrone and curdione from the essential oil of the rhizomes of Curcuma wenyujin by high-speed counter-current chromatography

AU - Yan, Jizhong

AU - Chen, Gang

AU - Tong, Shengqiang

AU - Feng, Yeping

AU - Sheng, Liuqing

AU - Lou, Jianzhong

PY - 2005/4/8

Y1 - 2005/4/8

N2 - High-speed counter-current chromatography (HSCCC) was applied to isolate and purify bioactive compounds in the essential oil of the rhizomes of Curcuma wenyujin. Two sesquiterpenes germacrone and curdione were successfully isolated and purified using two-phase solvent system composed of petroleum ether-ethanol-diethyl ether-water (5/4/0.5/1, v/v) in tail to head elution mode. 62 mg of germacrone and 93 mg of curdione were obtained from 658 mg of the essential oil each at over 95% purity. Their structures were identified by 1H NMR and EI MS.

AB - High-speed counter-current chromatography (HSCCC) was applied to isolate and purify bioactive compounds in the essential oil of the rhizomes of Curcuma wenyujin. Two sesquiterpenes germacrone and curdione were successfully isolated and purified using two-phase solvent system composed of petroleum ether-ethanol-diethyl ether-water (5/4/0.5/1, v/v) in tail to head elution mode. 62 mg of germacrone and 93 mg of curdione were obtained from 658 mg of the essential oil each at over 95% purity. Their structures were identified by 1H NMR and EI MS.

KW - Counter-current chromatography

KW - Curcuma wenyujin

KW - Curdione

KW - Germacrone

KW - Purification

UR - https://www.scopus.com/pages/publications/15744395815

U2 - 10.1016/j.chroma.2005.02.064

DO - 10.1016/j.chroma.2005.02.064

M3 - Article

SN - 0021-9673

VL - 1070

SP - 207

EP - 210

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Preparative isolation and purification of germacrone and curdione from the essential oil of the rhizomes of Curcuma wenyujin by high-speed counter-current chromatography

Abstract

Keywords

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