TY - JOUR
T1 - Preparative isolation and purification of harpagoside from Scrophularia ningpoensis Hemsley by high-speed counter-current chromatography
AU - Tong, Shenqiang
AU - Yan, Jizhong
AU - Lou, Jianzhong
PY - 2006/1/1
Y1 - 2006/1/1
N2 - The bioactive component harpagoside was successfully separated from the crude extract of Scrophularia ningpoensis Hemsley by one-step purification using high-speed counter-current chromatography (HSCCC). A two-phase solvent system containing n-butanol:ethyl acetate:water (1:9:10) was selected following consideration of the partition coefficient of the target compound. A 276 mg quantity of the crude extract was loaded onto a 250 mL HSCCC column and yielded 11 mg harpagoside at over 97% purity. The chemical structure of harpagoside was determined by HPLC-ESI/MS and 1H-NMR. Copyright © 2006 John Wiley & Sons, Ltd.
AB - The bioactive component harpagoside was successfully separated from the crude extract of Scrophularia ningpoensis Hemsley by one-step purification using high-speed counter-current chromatography (HSCCC). A two-phase solvent system containing n-butanol:ethyl acetate:water (1:9:10) was selected following consideration of the partition coefficient of the target compound. A 276 mg quantity of the crude extract was loaded onto a 250 mL HSCCC column and yielded 11 mg harpagoside at over 97% purity. The chemical structure of harpagoside was determined by HPLC-ESI/MS and 1H-NMR. Copyright © 2006 John Wiley & Sons, Ltd.
KW - Counter-current chromatography
KW - Harpagoside
KW - One-step purification
KW - Preparative chromatography
KW - Scrophularia ningpoensis Hemsley
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33750720631&origin=inward
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=33750720631&origin=inward
U2 - 10.1002/pca.938
DO - 10.1002/pca.938
M3 - Article
C2 - 17144248
SN - 0958-0344
VL - 17
SP - 406
EP - 408
JO - Phytochemical Analysis
JF - Phytochemical Analysis
IS - 6
ER -