Profiling of cell stress proteins reveals decreased expression of enzymatic antioxidants in tracheal epithelial tissue of pigs raised indoors

Exposure to indoor swine production facilities (SPF) environments causes airway inflammation and diseases including asthma, chronic bronchitis and chronic obstructive pulmonary disease (COPD) in facility workers. However, less is known about the impact of SPF exposures on the respiratory health of pigs. Respiratory symptoms are associated with repeat exposure to SPF, specifically inhalation of organic dust and other air pollutants therein. A thorough understanding of the molecular pathways regulated by SPF exposure is needed to understand airway inflammation and chronic inflammatory lung disease. The present study measured the expression of proteins associated with oxidative stress and antioxidant defenses in the tracheal epithelial tissues of pigs reared in SPF or on pasture. Proteome profiler cell stress arrays, western blotting and enzyme activity assays were utilized to measure protein expression and activity levels in tracheal epithelial tissue extracts of pigs. It was determined that pigs raised in SPF express significantly less enzymatic antioxidants, including superoxide dismutase (SOD), within their tracheal epithelial tissues compared to pasture raised pigs. Concomitantly, tracheal epithelial tissues of SPF raised pigs had lower SOD and catalase antioxidant activity levels compared to pasture raised pigs. The observations summarized herein provide evidence that exposure to swine production environments influence endogenous enzymatic antioxidant defenses within the tracheal epithelial tissues of pigs. This study offers insight for understanding the effect of continuous exposure to SPF pollutants on endogenous antioxidant defenses in the airway epithelial and may be helpful in understanding human airway responses to swine barn exposures.