TY - JOUR
T1 - Purification, characterization and antioxidant activity of polysaccharides from Flammulina velutipes residue
AU - Liu, Ying
AU - Zhang, Bin
AU - Ibrahim, SA
AU - Gao, Shuang-Shuang
AU - Yang, Hong
AU - Huang, Wen
PY - 2016/7/10
Y1 - 2016/7/10
N2 - In this study, we isolated polysaccharides from Flammulina velutipes residue (FVRP) using microwave-assisted extraction and then purified the polysaccharides by column chromatography to yield FVRP-1, FVRP-2 and FVRP-3. The structural characteristics of FVRP-1, FVRP-2 and FVRP-3 were investigated, and their antioxidant activities against ABTS+, DPPH and hydroxyl radicals were also analyzed in vitro. FVRP-1 was found to be neutral and rich in galactose. However, FVRP-2 and FVRP-3 were acidic polysaccharides and were rich in glucose. The average molecular weight of FVRP-1, FVRP-2 and FVRP-3 were 29,930, 62,290, and 36,310 Da, respectively. The glycosyl residue of FVRP-1 was an α-type glycosidic linkage, whereas FVRP-2 and FVRP-3 were β-type glycosidic linkages. We found FVRP-1, FVRP-2 and FVRP-3 had strong potential antioxidant activities in the order of FVRP-1 < FVRP-3 < FVRP-2. Our results thus suggested that FVRPs might be suitable for use as functional foods and as potential therapeutic agents.
AB - In this study, we isolated polysaccharides from Flammulina velutipes residue (FVRP) using microwave-assisted extraction and then purified the polysaccharides by column chromatography to yield FVRP-1, FVRP-2 and FVRP-3. The structural characteristics of FVRP-1, FVRP-2 and FVRP-3 were investigated, and their antioxidant activities against ABTS+, DPPH and hydroxyl radicals were also analyzed in vitro. FVRP-1 was found to be neutral and rich in galactose. However, FVRP-2 and FVRP-3 were acidic polysaccharides and were rich in glucose. The average molecular weight of FVRP-1, FVRP-2 and FVRP-3 were 29,930, 62,290, and 36,310 Da, respectively. The glycosyl residue of FVRP-1 was an α-type glycosidic linkage, whereas FVRP-2 and FVRP-3 were β-type glycosidic linkages. We found FVRP-1, FVRP-2 and FVRP-3 had strong potential antioxidant activities in the order of FVRP-1 < FVRP-3 < FVRP-2. Our results thus suggested that FVRPs might be suitable for use as functional foods and as potential therapeutic agents.
KW - Antioxidant activity
KW - Characterization
KW - Flammulina velutipes residue
KW - Polysaccharide
KW - Purification
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U2 - 10.1016/j.carbpol.2016.03.020
DO - 10.1016/j.carbpol.2016.03.020
M3 - Article
C2 - 27106153
SN - 0144-8617
VL - 145
SP - 71
EP - 77
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -