TY - JOUR
T1 - Reversible addition-fragmentation chain transfer polymerization in DNA biosensing
AU - He, Peng
AU - Zheng, Weiming
AU - Tucker, Eric Z.
AU - Gorman, Christopher B.
AU - He, Peng
PY - 2008/5/15
Y1 - 2008/5/15
N2 - Reversible addition-fragmentation chain transfer polymerization is employed here to allow detector-free visualization of specific DNA sequences for which dynamic polymer growth is used in signal amplification. In particular, surface-initiated polymer growth was regulated by the immobilization of chain transfer agents on the Au surface where DNA hybridization occurred. A linear polymer growth was observed as a function of the reaction time, characteristic of "living" polymer reactions. Significant improvement in assay sensitivity was realized in comparison to the previously reported polymerization-based sensing method by enhancing polymer growth rate and reducing background noises caused by nonspecific adsorption. Direct visualization of fewer than 2,000 copies of a short oligonucleotide sequence was demonstrated in a detector-free fashion. © 2008 American Chemical Society.
AB - Reversible addition-fragmentation chain transfer polymerization is employed here to allow detector-free visualization of specific DNA sequences for which dynamic polymer growth is used in signal amplification. In particular, surface-initiated polymer growth was regulated by the immobilization of chain transfer agents on the Au surface where DNA hybridization occurred. A linear polymer growth was observed as a function of the reaction time, characteristic of "living" polymer reactions. Significant improvement in assay sensitivity was realized in comparison to the previously reported polymerization-based sensing method by enhancing polymer growth rate and reducing background noises caused by nonspecific adsorption. Direct visualization of fewer than 2,000 copies of a short oligonucleotide sequence was demonstrated in a detector-free fashion. © 2008 American Chemical Society.
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U2 - 10.1021/ac702608k
DO - 10.1021/ac702608k
M3 - Article
C2 - 18416564
SN - 0003-2700
VL - 80
SP - 3633
EP - 3639
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 10
ER -